Wyatt-Hodge smashed her second century of the season but could not prevent Surrey from losing back-to-back matches.
Maia Bouchier (78) and Abi Norgrove (81 not out) had underpinned Hampshire’s 272 with half-centuries.
And Bex Tyson (3-60) proved the best of the three left-arm spinners on show, despite being the only one of the trio not selected for the T20 World Cup, by taking three wickets.
England star Wyatt-Hodge almost trumped the lot with a masterful 11th career hundred but Hampshire held their nerve to win by 13 runs to remain unbeaten and knock Surrey off the top of the table.
All eyes were on Tilly Corteen-Coleman after the 18-year-old had been picked in England’s T20 World Cup squad, along with the party to face New Zealand – her first international recognition.
She was immediately in the action as she bowled the first over after Hampshire had chosen to bat first, and proceeded to only go for nine runs in her first six-over spell.
The tone that Corteen-Coleman set allowed Surrey to pile on the pressure and stop Hampshire from ever looking like posting an imposing total, despite a host of starts from their top order.
Hampshire’s 272-5 had looked slightly short at half-way but Surrey also struggled to find fluency on the used pitch.
Paige Scholfield was the only top order Surrey batter to score at will, striking 44 off 55 balls, but her demise sparked a collapse of four wickets in 15 balls.
At 81-6 the damage appeared to have been done, with 192 still required, but Wyatt-Hodge had other ideas by using every bit of her experience.
But having looked on course to completing a heist, she was run out for 119 with 22 still needed from 15 balls – which Hampshire defended.